Characterisation of Magnetic Particles - DNA Interaction for Imobilisation of Viruses and Their Subsequent Identification by Zeta Potential and Particle Size Analyses
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43908591" target="_blank" >RIV/62156489:43210/15:43908591 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/15:PU126353
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Characterisation of Magnetic Particles - DNA Interaction for Imobilisation of Viruses and Their Subsequent Identification by Zeta Potential and Particle Size Analyses
Original language description
Magnetic nanoparticles offer several advantages for isolation of biological materials. Since they are broadly used in diagnostic immunoassays and in various reactions involving enzymes, proteins, and DNA, this study considers the interaction of four surface modified magnetic microparticles with selected DNA fragments. The magnetic microparticle precursors are prepared by reduction of from iron (III) nitrate and sodium borohydride in ammonia solution. Different surface modifications, named as MAN 37, MAN127, MAN 158 and MAN 164 were obtained by targeted chemical treatments. The zeta potential and particle size measurements were performed in a zetasizer MALVERN instrument. All considered magnetic microparticles are characterized by positive zeta-potentials, while the DNA fragments in solution show an approx. zeta-potential of -0.22 mV. All the particles were polycharged. MAN 37 and MAN 127 reveal the highest initial zeta potentials. Upon exposure to DNA, MAN 37 and MAN 127 particles low
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CC - Organic chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
NANOCON 2015: Conference Proceedings
ISBN
978-80-87294-63-5
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
505-510
Publisher name
Tanger Ltd.
Place of publication
Ostrava
Event location
Brno
Event date
Oct 14, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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