Age affects not only metabolome but also metal toxicity in Scenedesmus quadricauda cultures

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43909555" target="_blank" >RIV/62156489:43210/16:43909555 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/16:00090651 RIV/00216305:26620/16:PU120123
Result on the web
<a href="http://dx.doi.org/10.1016/j.jhazmat.2015.11.056" target="_blank" >http://dx.doi.org/10.1016/j.jhazmat.2015.11.056</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jhazmat.2015.11.056" target="_blank" >10.1016/j.jhazmat.2015.11.056</a>

Result language
angličtina
Original language name
Age affects not only metabolome but also metal toxicity in Scenedesmus quadricauda cultures
Original language description
Responses of Scenedesmus quadricauda grown in vitro and differing in age (old culture-13 months, young culture-1 month) to short-term cadmium (Cd) or nickel (Ni) excess (24 h) were compared. Higher age of the culture led to lower amount of chlorophylls, ascorbic acid and glutathione but higher signal of ROS. Surprisingly, sucrose was detected using DART-Orbitrap MS in both old and young culture and subsequent quantification confirmed its higher amount (ca. 3-times) in the old culture. Cd affected viability and ROS amount more negatively than Ni that could arise from excessive Cd uptake which was also higher in all treatments than in respective Ni counterparts. Surprisingly, nitric oxide was not extensively different in response to age or metals. Strong induction of phytochelatin 2 is certainly Cd-specific response while Ni also elevated ascorbate content. Krebs cycle acids were more accumulated in the young culture but they were rather elevated in the old culture (citric acid under Ni excess). We conclude that organic solid 'Milieu Bristol' medium we tested is suitable for long-term storage of unicellular green algae (also successfully tested for Coccomyxa sp. and Parachlorella sp.) and the impact of age on metal uptake may be useful for bioremediation purposes.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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