Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43909572" target="_blank" >RIV/62156489:43210/16:43909572 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/16:10323450 RIV/00216208:11130/16:10323450 RIV/00216224:14110/16:00091191 RIV/00216305:26620/16:PU120393 RIV/00064203:_____/16:10323450
Result on the web
<a href="http://dx.doi.org/10.1002/pros.23159" target="_blank" >http://dx.doi.org/10.1002/pros.23159</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/pros.23159" target="_blank" >10.1002/pros.23159</a>
Alternative languages
Result language
angličtina
Original language name
Relation of exposure to amino acids involved in sarcosine metabolic pathway on behavior of non-tumor and malignant prostatic cell lines
Original language description
BACKGROUNDSarcosine (N-methylglycine) was previously delineated as a substantial oncometabolite of prostate cancer (PCa) and its metabolism seems to be significantly involved in PCa development and behavior. METHODSWe focused on investigation whether the exposure of prostate cells (PNT1A, 22Rv1, and PC-3) to sarcosine-related amino acids (glycine, dimethylglycine, and sarcosine) affects their aggressiveness (cell mobility and division rates, using real-time cell based assay). The effect of supplementation on expression of glycine-N-methyltransferase (GNMT) mRNA was examined using qRT-PCR. Finally, post-treatment amino acids patterns were determined with consequent statistical processing using the Ward's method, factorial ANOVA and principal component analysis (P<0.05). RESULTSThe highest migration induced sarcosine and glycine in metastatic PC-3 cells (a decrease in relative free area about 53% and 73%). The highest cell division was achieved after treatment of 22Rv1 and PC-3 cells with sarcosine (time required for division decreased by 65% or 45%, when compared to untreated cells). qRT-PCR revealed also significant effects on expression of GNMT. Finally, amino acid profiling shown specific amino acid patterns for each cell line. In both, treated and untreated PC-3 cells significantly higher levels of serine, glutamic acid, and aspartate, linked with prostate cancer progression were found. CONCLUSIONSSarcosine-related amino acids can exceptionally affect the behavior of benign and malignant prostate cells.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA16-18917S" target="_blank" >GA16-18917S: The study of sarcosine metabolism and its participation in prostate cancer development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Prostate
ISSN
0270-4137
e-ISSN
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Volume of the periodical
76
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
679-690
UT code for WoS article
000373932700007
EID of the result in the Scopus database
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