Detection of DNA fragments from wheat in blood of animals

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43909622" target="_blank" >RIV/62156489:43210/16:43909622 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00003-016-1035-3" target="_blank" >http://dx.doi.org/10.1007/s00003-016-1035-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00003-016-1035-3" target="_blank" >10.1007/s00003-016-1035-3</a>

Result language

angličtina

Original language name

Detection of DNA fragments from wheat in blood of animals

Original language description

Delivery of foreign DNA is still a very controversial issue, especially in relation to genetically modified organisms. It is not necessary to use genetically modified feed to verify the possible transfer of DNA from food into the body. In this study, we evaluated the possible transfer of DNA from wheat forage (of single component diet) into the blood of three different organisms. DNA isolation kits were used to isolate total DNA from blood and feed. Blood was taken for analysis from rats (after 23 days feeding wheat), broilers (15 days), and carp (34 days). Housekeeping genes were used to verify blood samples (rats: glyceraldehyde-3-phosphate dehydrogenase/GAPDH/, broilers: growth hormone gene/GCH/and carp: small ribosomal subunit/40S/). We used GAPDH and actin to determine whether DNA from wheat was transferred into animal blood. No purple and common wheat DNA fragments were detected in the blood of any animal tested, suggesting no transfer of the tested wheat DNA into the body. Various contents of anthocyanins in the feeding variants had no effect on the transfer of wheat DNA into the blood of animals. Our results correspond to some studies that have not proved transfer DNA from genetically modified feed into the target organism.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal für Verbraucherschutz und Lebensmittelsicherheit

ISSN

1661-5751

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

3

Country of publishing house

CH - SWITZERLAND

Number of pages

6

Pages from-to

259-264

UT code for WoS article

000382704600006

EID of the result in the Scopus database

2-s2.0-84976439951