In Vivo Fluorescence Visualization of Quantum Dot Nanoparticles in Plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43910243" target="_blank" >RIV/62156489:43210/16:43910243 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26220/16:PU124274

Result on the web

<a href="https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

In Vivo Fluorescence Visualization of Quantum Dot Nanoparticles in Plants

Original language description

Visualization of nanoparticles can be exceedingly useful in tracking of targeted drug delivery systems. For this purpose, highly luminescent quantum dot nanoparticles may represent a suitable option due to their superior photophysical properties and versatile surface chemistry. This study was mainly focused on application of CdTe quantum dot nanoparticles for fluorescence imaging of their transport in plants. In vivo experiments were carried using leaves of sunflower plant (Helianthus annuus). Leaves, soaking water solution of CdTe-PVP and CdTe/ZnS quantum dots, were monitored (ʎem 535 nm and 600 nm respectively) for 8 hours at time intervals of 60 minutes using In Vivo Xtreme Imaging System (Bruker, MA, USA). Autofluorescence of biomolecules present in plants, including chlorophyll, carotene and xanthophylls, represents a crucial problem in fluorescence imaging of plants. However, by using adequate excitation and emission filters during fluorescence images acquisition, this phenomenon can be effectively suppressed. Moreover, multispectral imaging and spectral modelling can be performed in order to distinguish fluorescence of quantum dots. In this study, a comparison of two different modifications of CdTe quantum dots is provided together with recommendations on setting of appropriate excitation and emission range for image acquisition.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

MendelNet 2016: Proceedings of International PhD Students Conference

ISBN

978-80-7509-443-8

ISSN

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e-ISSN

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Number of pages

5

Pages from-to

1026-1030

Publisher name

Mendelova univerzita v Brně

Place of publication

Brno

Event location

Brno

Event date

Nov 9, 2016

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

000392968500184