Protein metabolism disruption and its effect on plant growth

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43911092" target="_blank" >RIV/62156489:43210/16:43911092 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Protein metabolism disruption and its effect on plant growth
Original language description
More than 6 % of the Arabidopsis genome encodes components of the ubiquitin conjugation system and, in effect, most plant regulatory circuitry, and many steps of process execution, depend on ubiquitin modification. Recent years have shown that plant hormones operate through circuits in which targeted, conditional turnover of regulator proteins via proteasome are of critical importance. Thus, a disruption of the ubiquitin-proteasome system (UPS) would have a significant impact on plant growth and development. Here we employed a potent proteasome inhibitor and a transgenic Arabidopsis line RV 86-5 carrying modified inducible ubiquitin that prevents protein degradation via the UPS. We followed the effects on plant physiology and analyzed proteome-wide responses via an LC-MS. The results present evidence that an intricate balance between the UPS and cytokinin modulates the growth response output of cytokinin signaling. On the proteome level, an LC-MS profiling identified >4,000 proteins, 61 of which were previously identified in response to proteasome inhibition.
Czech name
—
Czech description
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)