Apoferritin as an ubiquitous nanocarrier with excellent shelf life
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43911471" target="_blank" >RIV/62156489:43210/17:43911471 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/17:PU123312
Result on the web
<a href="https://doi.org/10.2147/IJN.S130267" target="_blank" >https://doi.org/10.2147/IJN.S130267</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2147/IJN.S130267" target="_blank" >10.2147/IJN.S130267</a>
Alternative languages
Result language
angličtina
Original language name
Apoferritin as an ubiquitous nanocarrier with excellent shelf life
Original language description
Due to many adverse effects of conventional chemotherapy, novel methods of targeting drugs to cancer cells are being investigated. Nanosize carriers are a suitable platform for this specific delivery. Herein, we evaluated the long-term stability of the naturally found protein nanocarrier apoferritin (Apo) with encapsulated doxorubicin (Dox). The encapsulation was performed using Apo's ability to disassemble reversibly into its subunits at low pH (2.7) and reassemble in neutral pH (7.2), physically entrapping drug molecules in its cavity (creating ApoDox). In this study, ApoDox was prepared in water and phosphate-buffered saline and stored for 12 weeks in various conditions (-20oC, 4oC, 20oC, and 37oC in dark, and 4oC and 20oC under ambient light). During storage, a very low amount of prematurely released drug molecules were detected (maximum of 7.5% for ApoDox prepared in PBS and 4.4% for ApoDox prepared in water). Fourier-transform infrared spectra revealed no significant differences in any of the samples after storage. Most of the ApoDox prepared in phosphate-buffered saline and ApoDox prepared in water and stored at -20oC formed very large aggregates (up to 487% of original size). Only ApoDox prepared in water and stored at 4oC showed no significant increase in size or shape. Although this storage caused slower internalization to LNCaP prostate cancer cells, ApoDox (2.5 µM of Dox) still retained its ability to inhibit completely the growth of 1.5x104 LNCaP cells after 72 hours. ApoDox stored at 20oC and 37oC in water was not able to deliver Dox inside the nucleus, and thus did not inhibit the growth of the LNCaP cells. Overall, our study demonstrates that ApoDox has very good stability over the course of 12 weeks when stored properly (at 4oC), and is thus suitable for use as a nanocarrier in the specific delivery of anticancer drugs to patients.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Nanomedicine
ISSN
1176-9114
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
24 March
Country of publishing house
NZ - NEW ZEALAND
Number of pages
14
Pages from-to
2265-2278
UT code for WoS article
000397274500001
EID of the result in the Scopus database
2-s2.0-85016330513