An enzymatic assay based on luciferase Ebola virus-like particles for evaluation of virolytic activity of antimicrobial peptides
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43911554" target="_blank" >RIV/62156489:43210/17:43911554 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/17:00096028 RIV/00216305:26620/17:PU123111
Result on the web
<a href="https://doi.org/10.1016/j.peptides.2016.12.015" target="_blank" >https://doi.org/10.1016/j.peptides.2016.12.015</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.peptides.2016.12.015" target="_blank" >10.1016/j.peptides.2016.12.015</a>
Alternative languages
Result language
angličtina
Original language name
An enzymatic assay based on luciferase Ebola virus-like particles for evaluation of virolytic activity of antimicrobial peptides
Original language description
Antimicrobial peptides are currently considered as promising antiviral compounds. Current assays to evaluate the effectivity of peptides against enveloped viruses based on liposomes or hemolysis are encumbered by the artificial nature of liposomes or distinctive membrane composition of used erythrocytes. We propose a novel assay system based on enzymatic Ebola virus-like particles containing sensitive luciferase reporter. The assay was validated with several cationic and anionic peptides and compared with lentivirus inactivation and hemolytic assays. The assay is sensitive and easy to perform in standard biosafety level laboratory with potential for high-throughput screens. The use of virus-like particles in the assay provides a system as closely related to the native viruses as possible eliminating some issues associated with other more artificial set ups. We have identified CAM-W (KWKLWKKIEKWGQGIGAVLKWLTTWL) as a peptide with the greatest antiviral activity against infectious lentiviral vectors and filoviral virus-like particles.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Peptides
ISSN
0196-9781
e-ISSN
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Volume of the periodical
88
Issue of the periodical within the volume
February
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
87-96
UT code for WoS article
000393930700012
EID of the result in the Scopus database
2-s2.0-85007411053