Size-related cytotoxicological aspects of polyvinylpyrrolidone-capped platinum nanoparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912017" target="_blank" >RIV/62156489:43210/17:43912017 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43410/17:43912017 RIV/00216305:26620/17:PU124250
Result on the web
<a href="https://doi.org/10.1016/j.fct.2017.04.043" target="_blank" >https://doi.org/10.1016/j.fct.2017.04.043</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.fct.2017.04.043" target="_blank" >10.1016/j.fct.2017.04.043</a>
Alternative languages
Result language
angličtina
Original language name
Size-related cytotoxicological aspects of polyvinylpyrrolidone-capped platinum nanoparticles
Original language description
The nanotechnological concept is based on size-dependent properties of particles in the 1-100 nm range. Nevertheless, the connection between their size and effect is still not clear. Thus, we focused on reductive colloidal synthesis, characterization and biological testing of Pt nanoparticles (PtNPs) capped with biocompatible polymer polyvinylpyrrolidone (PVP). Synthesized PtNPs were of 3 different primary sizes (approx. TILDE OPERATOR+D9110; TILDE OPERATOR+D9114 and > 20 nm) and demonstrated exceptional haemocompatibility. In vitro treatment of three different types of malignant cells (prostate - LNCaP, breast - MDA-MB-231 and neuroblastoma - GI-ME-N) revealed that even marginal differences in PtNPs diameter resulted in changes in their cytotoxicity. The highest cytotoxicity was observed using the smallest PtNPs-10, where 24IC50 was lower (3.1-6.2 μg/mL) than for cisplatin (8.1-19.8 μg/mL). In contrast to MDA-MB-231 and LNCaP cells, in GI-ME-N cells PtNPs caused noticeable changes in their cellular structure without influencing their viability. Post-exposure analyses revealed that PtNPs-29 and PtNPs-40 were capable of forming considerably higher amount of reactive oxygen species with consequent stimulation of expression of metallothionein (MT1/2 and MT3), at both mRNA and protein level. Overall, our pilot study demonstrates that in the nanoscaled world even the smallest differences can have crucial biological effect.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Food and Chemical Toxicology
ISSN
0278-6915
e-ISSN
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Volume of the periodical
105
Issue of the periodical within the volume
July
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
337-346
UT code for WoS article
000403625000035
EID of the result in the Scopus database
2-s2.0-85019049496