The effect of coffee supplementation on glutathione and total thiols levels

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912621" target="_blank" >RIV/62156489:43210/17:43912621 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/17:PU128347
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The effect of coffee supplementation on glutathione and total thiols levels
Original language description
Antioxidants are very important substances that counteract the formation of free radicals. They are divided into exogenous, such as vitamin C which the body receives with food, and endogenous. One of the most important endogenous antioxidants is glutathione (γ-glutamyl-L-cysteinyl-glycine), which plays an important role in cellular defense against oxidative damage. Free glutathione is presented within organisms in both reduced (GSH) and oxidized forms (GSSG). Oxidative stress leads to a decrease in GSH level and therefore a GSH/GSSG ratio that can be used as an indicator of oxidative stress and an indicator of various diseases. The aim of presented study was to develop a sampling method for capillary blood testing, where we have found that this amount (15 µl) is sufficient for our testing. Another aim was to supplement group of volunteers with coffee and to determine GSH levels and levels of total thiols after 0, 48 h and 96 h of supplementation in capillary blood. HPLC with electrochemical detection was used for GSH determination and Ellman's method for determination of total thiols. We could see the GSH slight increase as well as the levels of total thiols.
Czech name
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Czech description
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Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2017: Proceedings of International PhD Students Conference
ISBN
978-80-7509-529-9
ISSN
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e-ISSN
neuvedeno
Number of pages
6
Pages from-to
843-848
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 8, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000440194500151

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