Kinetic analysis of human metallothionein and CdTe quantum dot complexes using fluorescence and voltammetry techniques

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912864" target="_blank" >RIV/62156489:43210/17:43912864 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/17:PU124985

Result on the web

<a href="https://doi.org/10.1016/j.colsurfb.2017.09.033" target="_blank" >https://doi.org/10.1016/j.colsurfb.2017.09.033</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2017.09.033" target="_blank" >10.1016/j.colsurfb.2017.09.033</a>

Result language

angličtina

Original language name

Kinetic analysis of human metallothionein and CdTe quantum dot complexes using fluorescence and voltammetry techniques

Original language description

Thanks to quantum dots&apos; (QDs) properties, they can be used as selective and sensitive biomarkers in molecular imaging. In a previous paper, we confirmed the possibility of interaction between mercaptosuccinic acid-capped cadmium telluride QDs (MSA-CdTe) and human metallothionein (MT). The aim of this study was to expand on our previous research with an evaluation of the stability of the formed complexes between human MT and four CdTe compounds of the following sizes: 3.4 nm (blue QDs), 3.8 nm (green QDs), 4.5 nm (yellow QDs), and 5.2 nm (red QDs). Complexes were evaluated over time using fluorescence intensity and differential pulse voltammetry. Differences between the voltammograms obtained for standard solutions and for CdTe + MT show that complexes were formed. An increase in fluorescence intensity was observed for blue (Delta%approximate to 40 for t = 1 -&gt; 120 min) and red (Delta%approximate to 30 for t = 1 -&gt; 120 min) CdTe-MT complexes than CdTe alone, whereas green and yellow CdTe-MT complexes had a lower fluorescence intensity than CdTe alone. A stronger time dependence of the mercaptosuccinic acid (MSA) peak height on the timeline and differences in the MSA peak shape (in CdTe, and CdTe + MT complexes) were also observed by voltammetry. Authors noticed a decrease in the Cat2 signal of the red and green CdTe + MT complexes at the time of conjugation. Our results reveal that the size of QDs has an impact on the interaction between CdTe and human MT, as well as on the stability of complexes formed during these interactions. The bioconjugates&apos; stability was also found to depend on the time of interaction.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10403 - Physical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Colloids and Surfaces B. Biointerfaces

ISSN

0927-7765

e-ISSN

—

Volume of the periodical

160

Issue of the periodical within the volume

1 December

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

381-389

UT code for WoS article

000418978500046

EID of the result in the Scopus database

2-s2.0-85030109152