Sarcosine influences apoptosis and growth of prostate cells via cell-type specific regulation of distinct sets of genes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43912953" target="_blank" >RIV/62156489:43210/18:43912953 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/18:10374363 RIV/00216208:11310/18:10374363 RIV/00216305:26620/18:PU125946 RIV/00064203:_____/18:10374363

Result on the web

<a href="https://doi.org/10.1002/pros.23450" target="_blank" >https://doi.org/10.1002/pros.23450</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/pros.23450" target="_blank" >10.1002/pros.23450</a>

Result language

angličtina

Original language name

Sarcosine influences apoptosis and growth of prostate cells via cell-type specific regulation of distinct sets of genes

Original language description

Background: Sarcosine is a widely discussed oncometabolite of prostate cells. Although several reports described connections between sarcosine and various phenotypic changes of prostate cancer (PCa) cells, there is still a lack of insights on the complex phenomena of its effects on gene expression patterns, particularly in non-malignant and non-metastatic cells. Methods: To shed more light on this phenomenon, we performed parallel microarray profiling of RNA isolated from non-malignant (PNT1A), malignant (22Rv1), and metastatic (PC-3) prostate cell lines treated with sarcosine. Microarray results were experimentally verified using semi-quantitative-RT-PCR, clonogenic assay, through testing of the susceptibility of cells pre-incubated with sarcosine to anticancer agents with different modes of actions (inhibitors of topoisomerase II, DNA cross-linking agent, antimicrotubule agent and inhibitor of histone deacetylases) and by evaluation of activation of executioner caspases 3/7. Results: We identified that irrespective of the cell type, sarcosine stimulates up-regulation of distinct sets of genes involved in cell cycle and mitosis, while down-regulates expression of genes driving apoptosis. Moreover, it was found that in all cell types, sarcosine had pronounced stimulatory effects on clonogenicity. Except of an inhibitor of histone deacetylase valproic acid, efficiency of all agents was significantly (P&lt;0.05) decreased in sarcosine pre-incubated cells. Conclusions: Our comparative study brings evidence that sarcosine affects not only metastatic PCa cells, but also their malignant and non-malignant counterparts and induces very similar changes in cells behavior, but via distinct cell-type specific targets.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30204 - Oncology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Prostate

ISSN

0270-4137

e-ISSN

—

Volume of the periodical

78

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

104-112

UT code for WoS article

000417862300004

EID of the result in the Scopus database

2-s2.0-85037734681