Isolation of Reduced and Oxidized Glutathione Using Maghemite Particles

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43913352" target="_blank" >RIV/62156489:43210/18:43913352 - isvavai.cz</a>
Alternative codes found
RIV/00216305:26620/18:PU128336
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Isolation of Reduced and Oxidized Glutathione Using Maghemite Particles
Original language description
Here we report the optimal conditions for the isolation of reduced glutathione (GSH) and oxidized glutathione (GSSG) from various types of matrix, such as blood, plasma or cells and their characterization using ionexchange liquid chromatography with VIS detector. In our study, we aimed to synthesize paramagnetic microparticles able to isolate and immobilize GSH, GSSG from blood or plasma and thus preconcentrate it for subsequent analysis on ion-exchange liquid chromatography with VIS detector. We modified amberlite and Dowex by nanomaghemite (γ-Fe2O3), as the functional carriers providing perfect paramagnetic properties. Both the paramagnetic microparticles were able to bind GSH and GSSG. Our paramagnetic microparticles have potential to improve GSH and GSSG isolation and in future can serve for application as a platform of biosensors or delivery system.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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