Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914067" target="_blank" >RIV/62156489:43210/18:43914067 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/18:00103299 RIV/62156489:43410/18:43914067 RIV/00216305:26220/18:PU129333
Result on the web
<a href="https://doi.org/10.1016/j.envres.2018.06.024" target="_blank" >https://doi.org/10.1016/j.envres.2018.06.024</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.envres.2018.06.024" target="_blank" >10.1016/j.envres.2018.06.024</a>
Alternative languages
Result language
angličtina
Original language name
Effect of arsenic (III and V) on oxidative stress parameters in resistant and susceptible Staphylococcus aureus
Original language description
The presented study deals with the observation of properties of methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) in the toxic arsenic environment and influence of arsenic on antioxidant capacity. Two forms of arsenic (As(III), As(V)) with different concentrations were used for induction of the oxidative stress in tested strains. Microbiological methods showed that the growth inhibition of MSSA was higher than that of MRSA in presence of both arsenic ions. As(III) showed 24% and 33% higher anti-microbial effects than As(V) against MSSA and MRSA respectively. A similar result was found also in the experiment of reduction of biofilm-formation. By using spectrophotometry, it was revealed that As(III) induced higher antioxidant production in both bacterial cultures. Methicillin-susceptible S. aureus produced an app. 50 mg equivalent of gallic acid (GAE/1 mg of protein) and MRSA produced an app. 15 mg of GAE/1 mg of protein. The productions of metallothionein in MSSA and MRSA were decreased up to 62.41% and 55.84% respectively in presence of As ions. Reduction of As(III) and As(V) concentrations leads to a decrease in antioxidant production and increased the formation of metallothionein. All of these changes in the results were found to be significant statistically. Taken together, these experiments proved that in comparison with MSSA, MRSA is less susceptible not only to the antimicrobial effects of antibiotics but also against effects caused by metalloids, as arsenic. Thus, it can be stated that MRSA abounds with complex defensive mechanisms, which may in the future constitute significant problem in the efficiency of antibiotics alternatives as metal ions or nanoparticles.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/LO1401" target="_blank" >LO1401: Interdisciplinary Research of Wireless Technologies</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Environmental Research
ISSN
0013-9351
e-ISSN
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Volume of the periodical
166
Issue of the periodical within the volume
October
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
394-401
UT code for WoS article
000445318200042
EID of the result in the Scopus database
2-s2.0-85048838446