Isolation and detail characterization of aba1 T-DNA insertion mutant line of Arabidopsis thaliana
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914674" target="_blank" >RIV/62156489:43210/18:43914674 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Isolation and detail characterization of aba1 T-DNA insertion mutant line of Arabidopsis thaliana
Original language description
Abscisic acid is a plant hormone that affects many biological processes during plant lifespan including stomatal movement, seed and bud dormancy and organ size. Nevertheless, the role of abscisic acid in photomorphogenesis remains rudimentary. To study the role of abscisic acid in different light conditions, the characterization of homozygous mutant lines in biosynthetic gene encoding zeaxanthin epoxidase (ABA1) is essential for genetic studies. Here, we present isolation and characterization of one of the T-DNA insertion mutant lines of Arabidopsis thaliana ecotype Colombia-0, where the insertion is located in 5' UTR region (SALK_027326). We identified the homozygous plants from a segregating parental line by PCR. The corresponding PCR products were identical to those of a confirmed homozygous line (SALK_027326C). However, the ABA1 transcript abundance in isolated homozygous line was almost identical to the ABA1 transcript abundance in the wild-type plants, as was revealed by RT-qPCR analysis. Because the phenotype of the selected homozygous line was also similar to wildtype plants in response to a low light, we conclude that the 5' UTR mutation in the ABA1 gene does not affect the gene function and thus this line is not an eligible candidate for further genetic studies.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2018: Proceedings of International PhD Students Conference
ISBN
978-80-7509-597-8
ISSN
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e-ISSN
neuvedeno
Number of pages
5
Pages from-to
343-347
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 7, 2018
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000462205300064