A chemometric-assisted voltammetric analysis of free and Zn(II)-loaded metallothionein-3 states
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917686" target="_blank" >RIV/62156489:43210/20:43917686 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.bioelechem.2020.107501" target="_blank" >https://doi.org/10.1016/j.bioelechem.2020.107501</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bioelechem.2020.107501" target="_blank" >10.1016/j.bioelechem.2020.107501</a>
Alternative languages
Result language
angličtina
Original language name
A chemometric-assisted voltammetric analysis of free and Zn(II)-loaded metallothionein-3 states
Original language description
We focused on the application of mass spectrometry and electrochemical methods combined with a chemometric analysis for the characterization of partially metallothionein-3 species. The results showed decreased Cat1 and Cat2 signals for the Zn(II)-loaded MT3 species with respect to the metal-free protein, which might be explained by the arrangement of tetrahedral metal-thiolate coordination environments and the formation of metal clusters. Moreover, there was a decrease in the Cat1 and Cat2 signals, and a plateau was reached with 4-5 Zn(II) ions that corresponded to the formation of the C-terminal α-domain. Regarding the Zn7-xMT3 complexes, we observed three different electrochemical behaviours for the Zn1-2MT3, Zn3-6MT3 and Zn7MT3 species. The difference for Zn1-2MT3 might be explained by the formation of independent ZnS4 cores in this stage that differ with respect to the formation of ZnxCysy clusters with an increased Zn(II) loading. The binding of the third Zn(II) ion to MT3 resulted in high sample heterogeneity due the co-existence of Zn3-6MT3. Finally, the Zn7MT3 protein showed a third type of behaviour. The fact that there were no free Cys residues might explain this phenomenon. Thus, this research identifies the major proteins responsible for zinc buffering in the cell.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bioelectrochemistry
ISSN
1567-5394
e-ISSN
—
Volume of the periodical
134
Issue of the periodical within the volume
August
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
107501
UT code for WoS article
000579727300005
EID of the result in the Scopus database
2-s2.0-85082395904