Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917942" target="_blank" >RIV/62156489:43210/20:43917942 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/20:00118333 RIV/00216305:26620/20:PU136752
Result on the web
<a href="https://doi.org/10.3168/jds.2019-17367" target="_blank" >https://doi.org/10.3168/jds.2019-17367</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3168/jds.2019-17367" target="_blank" >10.3168/jds.2019-17367</a>

Result language
angličtina
Original language name
Molecularly imprinted polymers and capillary electrophoresis for sensing phytoestrogens in milk
Original language description
Dairy cow feed contains, among other ingredients, soybeans, legumes, and clover, plants that are rich in phytoestrogens. Several publications have reported a positive influence of phytoestrogens on human health; however, several unfavorable effects have also been reported. In this work, a simple, selective, and eco-friendly method of phytoestrogen isolation based on the technique of noncovalent molecular imprinting was developed. Genistein was used as a template, and dopamine was chosen as a functional monomer. A layer of molecularly imprinted polymers was created in a microtitration well plate. The binding capability and selective properties of obtained molecularly imprinted polymers were investigated. The imprinted polymers exhibited higher binding affinity toward chosen phytoestrogen than did the nonimprinted polymers. A selectivity factor of 6.94 was calculated, confirming satisfactory selectivity of the polymeric layer. The applicability of the proposed sensing method was tested by isolation of genistein from a real sample of bovine milk and combined with micellar electrokinetic capillary chromatography with UV-visible detection.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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