Analytical methods in strigolactone research
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917983" target="_blank" >RIV/62156489:43210/20:43917983 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15310/20:73603705 RIV/00027006:_____/20:10141687
Result on the web
<a href="https://doi.org/10.1186/s13007-020-00616-2" target="_blank" >https://doi.org/10.1186/s13007-020-00616-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13007-020-00616-2" target="_blank" >10.1186/s13007-020-00616-2</a>
Alternative languages
Result language
angličtina
Original language name
Analytical methods in strigolactone research
Original language description
Strigolactones (SLs) are important plant hormones that are produced via the carotenoid biosynthetic pathway and occur at extremely low concentrations in various plant species. They regulate root development, play important roles in symbioses between higher plants and mycorrhizal fungi, and stimulate germination of plant-parasitic Orobanche and Striga species. Chemical analysis is central to research on the biochemistry of SLs and their roles in developmental biology and plant physiology. Here we summarize key issues relating to the identification and quantification of SLs isolated from plant tissues and exudates. The advantages and drawbacks of different protocols used for strigolactone analysis are discussed, and guidelines for selecting a procedure that will minimize losses during isolation and purification prior to final analysis are proposed. Hyphenated techniques suitable for SL analysis such as GC-MS and LC-MS/MS are also discussed, and newer ambient techniques such as HR-DART-MS and DESI-MS are highlighted as tools with considerable potential in SL research. A key advantage of these methods is that they require only simply sample preparation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Methods
ISSN
1746-4811
e-ISSN
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Volume of the periodical
16
Issue of the periodical within the volume
29 May
Country of publishing house
GB - UNITED KINGDOM
Number of pages
13
Pages from-to
76
UT code for WoS article
000538846200001
EID of the result in the Scopus database
2-s2.0-85085611174