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Analytical methods in strigolactone research

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917983" target="_blank" >RIV/62156489:43210/20:43917983 - isvavai.cz</a>

  • Alternative codes found

    RIV/61989592:15310/20:73603705 RIV/00027006:_____/20:10141687

  • Result on the web

    <a href="https://doi.org/10.1186/s13007-020-00616-2" target="_blank" >https://doi.org/10.1186/s13007-020-00616-2</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1186/s13007-020-00616-2" target="_blank" >10.1186/s13007-020-00616-2</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Analytical methods in strigolactone research

  • Original language description

    Strigolactones (SLs) are important plant hormones that are produced via the carotenoid biosynthetic pathway and occur at extremely low concentrations in various plant species. They regulate root development, play important roles in symbioses between higher plants and mycorrhizal fungi, and stimulate germination of plant-parasitic Orobanche and Striga species. Chemical analysis is central to research on the biochemistry of SLs and their roles in developmental biology and plant physiology. Here we summarize key issues relating to the identification and quantification of SLs isolated from plant tissues and exudates. The advantages and drawbacks of different protocols used for strigolactone analysis are discussed, and guidelines for selecting a procedure that will minimize losses during isolation and purification prior to final analysis are proposed. Hyphenated techniques suitable for SL analysis such as GC-MS and LC-MS/MS are also discussed, and newer ambient techniques such as HR-DART-MS and DESI-MS are highlighted as tools with considerable potential in SL research. A key advantage of these methods is that they require only simply sample preparation.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10609 - Biochemical research methods

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Plant Methods

  • ISSN

    1746-4811

  • e-ISSN

  • Volume of the periodical

    16

  • Issue of the periodical within the volume

    29 May

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    13

  • Pages from-to

    76

  • UT code for WoS article

    000538846200001

  • EID of the result in the Scopus database

    2-s2.0-85085611174