Culicoidibacter larvae gen. Nov., sp. nov., from the gastrointestinal tract of the biting midge (culicoides sonorensis) larva, belongs to a novel lineage culicoidibacteraceae fam. nov., culicoidibacterales ord. nov. and culicoidibacteria classis nov. of the phylum firmicutes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43918915" target="_blank" >RIV/62156489:43210/20:43918915 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/20:43879034
Result on the web
<a href="https://doi.org/10.1099/ijsem.0.004543" target="_blank" >https://doi.org/10.1099/ijsem.0.004543</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1099/ijsem.0.004543" target="_blank" >10.1099/ijsem.0.004543</a>
Alternative languages
Result language
angličtina
Original language name
Culicoidibacter larvae gen. Nov., sp. nov., from the gastrointestinal tract of the biting midge (culicoides sonorensis) larva, belongs to a novel lineage culicoidibacteraceae fam. nov., culicoidibacterales ord. nov. and culicoidibacteria classis nov. of the phylum firmicutes
Original language description
Strain CS-1T, a novel facultative anaerobic bacterium, was isolated from the larval gastrointestinal tract of the biting midge, Culicoides sonorensis, a vector of the epizootic haemorrhagic disease virus and the bluetongue virus. Cells were Gram-stain-positive, non-motile, non-spore-forming, pleomorphic rods. Optimal growth occurred at pH 7.5 and 37 oC. The G+C content of the genomic DNA was 38.3 mol%, estimated by using HPLC. The dominant cellular fatty acids were C14: 0 (45.9 %) and C16: 0 (26.6 %). The polar lipid profile comprised glycolipids, diphosphatidylglycerol, phospholipids and phosphoglycolipids. Respira-tory quinones were not detected. Strain CS-1T had very low 16S rRNA gene similarity to members of the phylum Firmicutes: Macrococcus canis KM45013T (85 % similarity) and Turicibacter sanguinis MOL361T (88 % similarity). Phylogenetic analysis based on 16S rRNA, rpoB, gyrB genes, and conserved protein sequences of the whole genome revealed that strain CS-1T was related to members of the classes Bacilli and Erysipelotrichia within the phylum Firmicutes. Furthermore, average nucleotide identity and digital DNA-DNA hybridization analyses of the whole genome revealed very low sequence similarity to species of Bacilli and Erysipelotrichaceae (Macrococcus canis KM45013T and Turicibacter sp. H121). These results indicate that strain CS-1T belongs to the phylum Firmicutes and represents a new species of a novel genus, family, order and class. Based on the phenotypic, chemo-taxonomic, phylogenetic and genomic characteristics, we propose the novel taxon Culicoidibacter larvae gen. nov., sp. nov. with the type strain CS-1T (=CCUG 71726T=DSM 106607T) within the hereby new proposed novel family Culicoidibacteraceae fam. nov., new order Culicoidibacaterales ord. nov. and new class Culicoidibacteria classis nov. in the phylum Firmicutes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Systematic and Evolutionary Microbiology
ISSN
1466-5026
e-ISSN
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Volume of the periodical
70
Issue of the periodical within the volume
12
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
6482-6490
UT code for WoS article
000608418900057
EID of the result in the Scopus database
2-s2.0-85098257503