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Culicoidibacter larvae gen. Nov., sp. nov., from the gastrointestinal tract of the biting midge (culicoides sonorensis) larva, belongs to a novel lineage culicoidibacteraceae fam. nov., culicoidibacterales ord. nov. and culicoidibacteria classis nov. of the phylum firmicutes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43918915" target="_blank" >RIV/62156489:43210/20:43918915 - isvavai.cz</a>

  • Alternative codes found

    RIV/62157124:16810/20:43879034

  • Result on the web

    <a href="https://doi.org/10.1099/ijsem.0.004543" target="_blank" >https://doi.org/10.1099/ijsem.0.004543</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1099/ijsem.0.004543" target="_blank" >10.1099/ijsem.0.004543</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Culicoidibacter larvae gen. Nov., sp. nov., from the gastrointestinal tract of the biting midge (culicoides sonorensis) larva, belongs to a novel lineage culicoidibacteraceae fam. nov., culicoidibacterales ord. nov. and culicoidibacteria classis nov. of the phylum firmicutes

  • Original language description

    Strain CS-1T, a novel facultative anaerobic bacterium, was isolated from the larval gastrointestinal tract of the biting midge, Culicoides sonorensis, a vector of the epizootic haemorrhagic disease virus and the bluetongue virus. Cells were Gram-stain-positive, non-motile, non-spore-forming, pleomorphic rods. Optimal growth occurred at pH 7.5 and 37 oC. The G+C content of the genomic DNA was 38.3 mol%, estimated by using HPLC. The dominant cellular fatty acids were C14: 0 (45.9 %) and C16: 0 (26.6 %). The polar lipid profile comprised glycolipids, diphosphatidylglycerol, phospholipids and phosphoglycolipids. Respira-tory quinones were not detected. Strain CS-1T had very low 16S rRNA gene similarity to members of the phylum Firmicutes: Macrococcus canis KM45013T (85 % similarity) and Turicibacter sanguinis MOL361T (88 % similarity). Phylogenetic analysis based on 16S rRNA, rpoB, gyrB genes, and conserved protein sequences of the whole genome revealed that strain CS-1T was related to members of the classes Bacilli and Erysipelotrichia within the phylum Firmicutes. Furthermore, average nucleotide identity and digital DNA-DNA hybridization analyses of the whole genome revealed very low sequence similarity to species of Bacilli and Erysipelotrichaceae (Macrococcus canis KM45013T and Turicibacter sp. H121). These results indicate that strain CS-1T belongs to the phylum Firmicutes and represents a new species of a novel genus, family, order and class. Based on the phenotypic, chemo-taxonomic, phylogenetic and genomic characteristics, we propose the novel taxon Culicoidibacter larvae gen. nov., sp. nov. with the type strain CS-1T (=CCUG 71726T=DSM 106607T) within the hereby new proposed novel family Culicoidibacteraceae fam. nov., new order Culicoidibacaterales ord. nov. and new class Culicoidibacteria classis nov. in the phylum Firmicutes.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10606 - Microbiology

Result continuities

  • Project

    <a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    International Journal of Systematic and Evolutionary Microbiology

  • ISSN

    1466-5026

  • e-ISSN

  • Volume of the periodical

    70

  • Issue of the periodical within the volume

    12

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    9

  • Pages from-to

    6482-6490

  • UT code for WoS article

    000608418900057

  • EID of the result in the Scopus database

    2-s2.0-85098257503