Novel method for morphometry analysis of bursa of Fabricius
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919512" target="_blank" >RIV/62156489:43210/20:43919512 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Novel method for morphometry analysis of bursa of Fabricius
Original language description
Morphological observation of bursa of Fabricius (BF) represent a valuable tool in evaluation of stress causative agents on poultry. The main goal of this study was to compare two methods for morphometry analysis of BF: standard hematoxylin/eosin (HE/EO) staining and immunofluorescence staining based on anti-basal cell cytokeratin antibody with DAPI background staining (CYT/DAPI). Chickens were divided into two groups (18 for HE/EO and 18 for CYT/DAPI). BF from 6 chickens from each group were taken in 7, 14 and 22 days of age. In every chicken, 40 randomly selected follicles were evaluated. In total, comparison of data from all days between groups didn't show significant differences. However, age have a significant impact. In 14 days of age, size of the follicle and cortex were significantly higher in CYT/DAPI group (P<0.05). Additionally, in 22 days old chickens size of the medulla was significantly higher in HE/EO group. Cortex/medulla ratio at age 7 days was significantly higher in HE/EO (P<0.05), but in older chickens were significantly higher (P<0.05) in CYT/DAPI group. Immunofluorescence staining seems to be more exact method and should be used, if precise localization of cortico-medullary border is necessary. On the other hand, HE/EO staining is a better choice for quantitative analyses of higher number of samples.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2020: Proceedings of International PhD Students Conference
ISBN
978-80-7509-765-1
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
439-443
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 11, 2020
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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