Genetic analysis of selected genes active in chickpea seed dormancy and germination testing

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919581" target="_blank" >RIV/62156489:43210/20:43919581 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Genetic analysis of selected genes active in chickpea seed dormancy and germination testing
Original language description
The most important event in the plant life cycle is transition from seed to seedling. Germination is an irreversible process and therefore correct timing is required. Several dormancy mechanisms exist among plants. Physical dormancy can be observed in legumes. Recombinant inbred lines derived from crossing of cultivated and wild parents of chickpea serve as a good model to clarify seed dormancy loss during crop domestication and its genetic basis. Restriction analysis was carried out using the cleaving of amplified polymorphic sequences method. For 6 candidate loci located on intervals of chromosome 1 and 5 were determined possible primer combinations with products that showed variability over non-coding regions. Polymorphic restriction recognition sites were found and restriction mapping was done with parental genotypes. Testing of optimal conditions for restriction analysis and primer combinations for population mapping are still ongoing process. In addition, the presence of physical dormancy in seeds was observed during germination testing. Two parental genotypes and population of 51 inbred lines were evaluated over 7 days' period. Final germination percentage ranged from 20-100% and divided population into dormant and non-dormant group.
Czech name
—
Czech description
—

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2020: Proceedings of International PhD Students Conference
ISBN
978-80-7509-765-1
ISSN
—
e-ISSN
—
Number of pages
6
Pages from-to
390-395
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 11, 2020
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
—

Similar results(10)