Identification of volatile compounds associated with sepsis progression by mass spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919610" target="_blank" >RIV/62156489:43210/20:43919610 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Identification of volatile compounds associated with sepsis progression by mass spectrometry
Original language description
Herein, SPMEMINUS SIGN GCxGCMINUS SIGN TOFMS method was used to optimize the parameters of the method and correct conditions for sample processing which will allow to develop a method for the identification of VOCs (mainly alcohol, ketones and hydrocarbons) sepsis biomarkers. The correct conditions for processing the samples (the incubation - heating/collection of samples, the selection of SPME fiber for sample collection and the addition of NaCl) were established. Moreover, in this work, the GCMINUS SIGN MS (Pegasus 4DMINUS SIGN C) method was used. Parameters of the chromatography and mass spectrometry methods GCxGCMINUS SIGN TOFMS were tested and optimized (temperature of the secondary oven, modulator temperature, modulator period, cool time etc.). Altogether, about 5 methods with different settings were compared. Finally, the best combination of the optimized sample conditions and optimized method was developed. The heating and collection of the sample 30min/30min by the DVB/CAR/PDMS fiber in the addition of NaCl (200 mg/ml) were chosen. Moreover, one of the 5 optimized GCxGCMINUS SIGN TOFMS method was selected. The optimized method will be used for analyses of monocyte from serum samples.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2020: Proceedings of International PhD Students Conference
ISBN
978-80-7509-765-1
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
548-552
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 11, 2020
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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