Optimization of Metafectene-mediated transfection of metallothionein down-regulating siRNA in triple-negative breast cancer cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919611" target="_blank" >RIV/62156489:43210/20:43919611 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Optimization of Metafectene-mediated transfection of metallothionein down-regulating siRNA in triple-negative breast cancer cells
Original language description
Small interfering RNA (siRNA) has become an important instrument for studying gene functions. These double strand RNA oligonucleotides are characterized by targeted mRNA degradation after introduction of specific sequence RNA. Efficiency of siRNA is the basis of gene silencing and gene therapy. In this case, we optimized siRNA transfection to achieve down regulation of gene expression. Then, we validated this optimization on specific sub isoform of metallothionein, MT-1X, into triple-negative breast carcinoma cell lines. In this project, we focused on achieving results with the best possible targeted gene suppression with high viability of cells and high transfection effectivity.
Czech name
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Czech description
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Project
<a href="/en/project/GC19-13766J" target="_blank" >GC19-13766J: Zinc signaling and metallothioneins sub/isoforms expression in breast cancer: implications for prognostic and therapeutic purposes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2020: Proceedings of International PhD Students Conference
ISBN
978-80-7509-765-1
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
553-556
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 11, 2020
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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