Immune response of rainbow trout (Oncorhynchus mykiss) fed with feed enriched by autochtonous probiotic lactobacilli

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43920412" target="_blank" >RIV/62156489:43210/21:43920412 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Immune response of rainbow trout (Oncorhynchus mykiss) fed with feed enriched by autochtonous probiotic lactobacilli

Original language description

Our aim was to study effect of feed that has been enriched with autochthonous probiotic Lactobacillus plantarum R2 on relative gene expression of immunologically important molecules in rainbow trout. This is a partial work and samples are still being analyzed. Experimental fish were divided into three groups of 10 individuals and each group was separated in its tank. First and second group received our probiotic feed; first group continually during whole experiment and second group cyclically. Third was control group, thus it received just commercial feed. After 7 weeks, samples of distal intestine were collected and change in relative gene expression was measured. Molecules that were studied were interleukin 1, interleukin 8, CD4, CD8 and immunoglobulin M. The gene expression was assessed using quantitative real time PCR with β-actin as a reference gene. Relative expression of interleukin 1 and 8 did not change significantly although interleukin 8 showed decreased expression in both experimental groups as compared to control. Expression of CD4 was increased in both experimental groups with significant change in continually fed group. CD8 showed slight increase in continual group and slight decrease in cyclical group. Slight increase of expression for immunoglobulin M was also measured in both experimental groups. Overall, lower expression for genes in cyclically fed group might be result of temporary change in feed that stressed fish. We conclude that continually administered probiotic feed positively affected immunity of fish by enhancing numbers of CD4 and CD8 cells and immunoglobulin M while not triggering inflammatory response.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40103 - Fishery

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NutriNET 2021: Proceedings of reviewed scientific papers

ISBN

978-80-8077-713-5

ISSN

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e-ISSN

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Number of pages

10

Pages from-to

96-105

Publisher name

Univerzita veterinárskeho lekárstva a farmácie v Košiciach

Place of publication

Košice

Event location

Košice

Event date

Sep 9, 2021

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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