Method for simultaneous detection of Phytophthora infestans proteins and DNA in Solanum tuberosum samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43921815" target="_blank" >RIV/62156489:43210/21:43921815 - isvavai.cz</a>

Result on the web

<a href="https://mendelnet.cz/media/mnet_2021_full.pdf" target="_blank" >https://mendelnet.cz/media/mnet_2021_full.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Method for simultaneous detection of Phytophthora infestans proteins and DNA in Solanum tuberosum samples

Original language description

Phytophthora infestans is a model organism for the oomycetes, a distinct lineage of funguslike eukaryotes. However, P. infestans is primarily known as a pathogen that causes the serious potato and tomato disease known as late blight. The means of controlling late blight are limited and require early detection of the pathogen. One of the most reliable method for targeted quantitation is based on qPCR. The amount of sample is often limited, which does not allow the use of other analyses such as proteome analysis by LC-MS. Here, we describe the optimized extraction method for simultaneously extracting the DNA and proteins from an infected leaf of Solanum tuberosum. Simultaneous analysis of the amount of P. infestans DNA and the proteome composition from the infected leaf opens possibilities for a better understanding of the interaction between P. infestans and the plant.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/QK1910045" target="_blank" >QK1910045: Identification of metabolites correlating with quantitative resistance to Phytophthora infestans</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

MendelNet 2021: Proceedings of International PhD Students Conference

ISBN

978-80-7509-821-4

ISSN

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e-ISSN

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Number of pages

5

Pages from-to

337-341

Publisher name

Mendelova univerzita v Brně

Place of publication

Brno

Event location

Brno

Event date

Nov 10, 2021

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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