Multilocus Sequence Genotype Heterogeneity in Streptococcus uberis Isolated from Bovine Mastitis in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F22%3A43921981" target="_blank" >RIV/62156489:43210/22:43921981 - isvavai.cz</a>

Alternative codes found

RIV/00027162:_____/22:N0000088 RIV/00216224:14310/22:00127387

Result on the web

<a href="https://doi.org/10.3390/ani12182327" target="_blank" >https://doi.org/10.3390/ani12182327</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ani12182327" target="_blank" >10.3390/ani12182327</a>

Result language

angličtina

Original language name

Multilocus Sequence Genotype Heterogeneity in Streptococcus uberis Isolated from Bovine Mastitis in the Czech Republic

Original language description

The ubiquitous occurrence and high heterogeneity of Streptococcus uberis strains cause difficulties in the development and implementation of effective control strategies in dairy herds. In this study, S. uberis strains from 74 farms, obtained predominantly from subclinical, acute, and chronic recurrent mastitis, as well as from udder surface swabs and milk from healthy udders, were analysed for their genetic diversity using multilocus sequence typing (MLST). Isolates were tested for the presence of the genes encoding the virulence factors using polymerase chain reaction. Antibiotic susceptibility testing was performed using a microdilution assay including 14 antimicrobials. The virulence profiles and antimicrobial (AMR) profiles of the isolates were assembled and the overall heterogeneity was evaluated. Among the 124 isolates, 89 MLST genotypes, 7 different virulence profiles, and 12 AMR profiles were identified. The large number of different MLST allelic profiles in this study points to the high heterogeneity of strains in dairy herds in the Czech Republic. Isolates of a certain MLST genotype may possess a different set of virulence factor genes. We detected up to three different resistance profiles within a single MLST genotype. The results of our study showed that fully susceptible isolates coexisted with resistant or even multiresistant isolates in the same herd. Multiple genotypes within a herd were detected on many farms (up to seven MLST genotypes and four AMR profiles in one herd). This heterogenic population structure might suggest that environmental transmission is the predominant route of infection in herds in the Czech Republic.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

<a href="/en/project/QK1910212" target="_blank" >QK1910212: Modern methods of diagnosis, therapy and prevention of mastitis caused by Streptococcus uberis as a tool for designing targeted control programs in cows</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Animals

ISSN

2076-2615

e-ISSN

2076-2615

Volume of the periodical

12

Issue of the periodical within the volume

18

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

2327

UT code for WoS article

000858175100001

EID of the result in the Scopus database

2-s2.0-85138510257