Comparison of cell death rate in various chicken lymphocytes populations in a response to three temperature treatments

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F23%3A43923926" target="_blank" >RIV/62156489:43210/23:43923926 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.55251/jmbfs.10306" target="_blank" >https://doi.org/10.55251/jmbfs.10306</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.55251/jmbfs.10306" target="_blank" >10.55251/jmbfs.10306</a>

Result language

angličtina

Original language name

Comparison of cell death rate in various chicken lymphocytes populations in a response to three temperature treatments

Original language description

The goal of the experiment was to evaluate susceptibility of lymphocytes populations derived from bursa of Fabricius, spleen, thymus, and blood to temperature treatments in vitro. Temperatures used in study was 37 &amp; DEG;C (thermoneutral), 41 &amp; DEG;C (mild heat stress) and 45 &amp; DEG;C (severe heat stress). Cells were incubated for 8 hours and after 3 and 8 hours were measured numbers of apoptotic, necrotic and CellROX positive cells by flow cytometry. Representation of live lymphocyte phenotypes (CD3, CD4, CD8, BU1) in cell populations were also measured. At all temperature treatments, thymus lymphocytes population had the lowest (p &lt; 0.05) percentage of apoptotic cells. On the other hand, significantly highest numbers (p &lt; 0.05) of apoptotic cells were found in bursal lymphocytes after 8 hours of incubation. Differences in numbers of necrotic cells were more pronounced at 45 &amp; DEG;C, where after 3 and 8 hours of incubation were significantly highest numbers (p &lt; 0.05) of necrotic cells in spleen. Increasing temperature induce production of reactive oxygen species (ROS) in cells and highest proportion (p &lt; 0.05) of ROS-positive cells were found in bursal and spleen cell population after 8 hours of incubation. Transmission electron microscopy showed loss of mitochondrial matrix and disrupted mitochondrial membrane in mitochondria of lymphocytes at 45 &amp; DEG;C as initial damage caused by ROS. From individual cell phenotypes, there were significantly decreased (p &lt; 0.05) B lymphocytes in bursa of Fabricius, CD4+ and CD8+ lymphocytes in thymus and whole T lymphocytes population in spleen at 45 &amp; DEG;C. Various susceptibility to temperature can be predictive factor of worse function in some organs in stress conditions where body temperature increase.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Microbiology, Biotechnology and Food Sciences

ISSN

1338-5178

e-ISSN

1338-5178

Volume of the periodical

13

Issue of the periodical within the volume

3

Country of publishing house

SK - SLOVAKIA

Number of pages

6

Pages from-to

"e10306"

UT code for WoS article

001047828800001

EID of the result in the Scopus database

2-s2.0-85179962752