Xylem Sap Proteome Analysis Provides Insight into Root–Shoot Communication in Response to flg22
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F24%3A43925506" target="_blank" >RIV/62156489:43210/24:43925506 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.3390/plants13141983" target="_blank" >https://doi.org/10.3390/plants13141983</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/plants13141983" target="_blank" >10.3390/plants13141983</a>
Alternative languages
Result language
angličtina
Original language name
Xylem Sap Proteome Analysis Provides Insight into Root–Shoot Communication in Response to flg22
Original language description
Xylem sap proteomics provides crucial insights into plant defense and root-to-shoot communication. This study highlights the sensitivity and reproducibility of xylem sap proteome analyses, using a single plant per sample to track over 3000 proteins in two model crop plants, Solanum tuberosum and Hordeum vulgare. By analyzing the flg22 response, we identified immune response components not detectable through root or shoot analyses. Notably, we discovered previously unknown elements of the plant immune system, including calcium/calmodulin-dependent kinases and G-type lectin receptor kinases. Despite similarities in the metabolic pathways identified in the xylem sap of both plants, the flg22 response differed significantly: S. tuberosum exhibited 78 differentially abundant proteins, whereas H. vulgare had over 450. However, an evolutionarily conserved overlap in the flg22 response proteins was evident, particularly in the CAZymes and lipid metabolism pathways, where lipid transfer proteins and lipases showed a similar response to flg22. Additionally, many proteins without conserved signal sequences for extracellular targeting were found, such as members of the HSP70 family. Interestingly, the HSP70 response to flg22 was specific to the xylem sap proteome, suggesting a unique regulatory role in the extracellular space similar to that reported in mammalians.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/8X23011" target="_blank" >8X23011: Identification of defense mechanisms against harmful and aggressive pathogens in economically important woody plants</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plants
ISSN
2223-7747
e-ISSN
2223-7747
Volume of the periodical
13
Issue of the periodical within the volume
14
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
1983
UT code for WoS article
001277253200001
EID of the result in the Scopus database
2-s2.0-85199911052