Evaluating DNA quality in Coleoptera and Lepidoptera: Impact of fixation and preservation in various trapping methods
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F25%3A43927038" target="_blank" >RIV/62156489:43210/25:43927038 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43410/25:43927038
Result on the web
<a href="https://doi.org/10.1111/eea.13591" target="_blank" >https://doi.org/10.1111/eea.13591</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/eea.13591" target="_blank" >10.1111/eea.13591</a>
Alternative languages
Result language
angličtina
Original language name
Evaluating DNA quality in Coleoptera and Lepidoptera: Impact of fixation and preservation in various trapping methods
Original language description
Despite advancements in barcoding and metabarcoding, preserving high-quality DNA from field-collected arthropods remains challenging. Although various fixatives and preservatives are used for DNA recovery in Coleoptera (Carabidae) and Lepidoptera (Noctuidae, Nolidae, Geometridae, and Tortricidae), their effects on DNA quality across trapping methods are not fully understood. This study evaluates fixation and preservation strategies affecting DNA integrity, focusing on pH changes before and after tissue grinding to improve consistency. For Carabidae, Calathus fuscipes (L.) were collected with a Malaise trap, while Platynus assimilis (Paykull) were collected via emergence traps and pitfall traps (with and without roof), using propylene glycol as a fixative. Preservation methods included storage in propylene glycol, 96% ethanol, or drying, with samples kept at MINUS SIGN 20oC for 1 year. Propylene glycol samples were washed with distilled water prior to grinding. Additional fixatives in individual trapping included ethylene glycol, propylene glycol, ethanol, brine, ethyl acetate, vinegar, and drying (with and without silica gel), stored at MINUS SIGN 20oC for 3 months. For Lepidoptera, specimens were categorized by size: large-Agrostis exclamationis (L.) (Noctuidae), medium-Meganola strigula (Denis et Schiffermüller) (Nolidae), Eupithecia insigniata (Hübner) (Geometridae), and small-Pelochrista caecimaculana (Hübner) (Tortricidae). Specimens were treated with chloroform (vapor and soaked) or cyanide vapors and stored at room temperature for 3 months. DNA quality was assessed through fragmentation analysis and PCR amplification of COI fragments (658, 313, and 157 bp for Coleoptera and 658, 311, and 220 bp for Lepidoptera) with Sanger sequencing. Results showed reduced DNA integrity in diluted Malaise trap samples, while distilled water washing improved readability in emergence trap samples. Brine proved a cost-effective preservative. For Lepidoptera, DNA preservation depended on sample size and fixative, with small chloroform-soaked specimens yielding non-sequencable DNA, while vapor-treated samples remained sequencable. This study offers insights to optimize DNA yield and preservation for arthropod research.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10616 - Entomology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Entomologia Experimentalis et Applicata
ISSN
0013-8703
e-ISSN
1570-7458
Volume of the periodical
173
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
903-917
UT code for WoS article
001485291700001
EID of the result in the Scopus database
2-s2.0-105004838576