Exogenously applied putrescine and chitosan–putrescine nanocomposite alleviate the negative effects of chilling stress on iceberg lettuce seedlings

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F25%3A43927355" target="_blank" >RIV/62156489:43210/25:43927355 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1038/s41598-025-11180-x" target="_blank" >https://doi.org/10.1038/s41598-025-11180-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-025-11180-x" target="_blank" >10.1038/s41598-025-11180-x</a>

Result language

angličtina

Original language name

Exogenously applied putrescine and chitosan–putrescine nanocomposite alleviate the negative effects of chilling stress on iceberg lettuce seedlings

Original language description

Climate change-induced stressors such as extreme temperatures negatively affect plant growth and development. Therefore, methods are needed to improve plant tolerance to abiotic stresses. The aim of this study was to evaluate whether the foliar application of putrescine (Put) and a chitosan-putrescine nanocomposite (Ch-Put) can modulate the antioxidant response and membrane properties of lettuce under chilling stress. In this study, Put and Ch-Put were applied via foliar spraying at two concentrations (1 mM and 2.5 mM), and after treatment, the plants were placed in a phytotron at 4 oC and 20 oC. Changes in the properties of the cell membranes were assessed in the seedlings. Additionally, the antioxidant enzymatic activity and content of nonenzymatic bioactive compounds were evaluated. The results indicated that the use of Put and Ch-Put influenced the permeability and fluidity of the lipid membranes, which also depended on the treatment temperature. An increase in Alim (all treatments) and a decrease in Cs-1 values (Put and Ch-Put at a concentration of 2.5 mM) were observed at 4 oC, suggesting looser packing and increased elasticity of cell membranes, facilitating the metabolic and physiological adaptation of plants to stress. The treatment of chilled plants with Put and Ch-Put resulted in increased contents of proline, carbohydrates, glutathione, phenolics and L-ascorbic acid, as well as increased activity of several antioxidant enzymes, such as catalase (CAT) and ascorbate peroxidase (APX), which could contribute to increased tolerance to stress. The strongest effects were observed for Put at concentrations of 1 mM and 2.5 mM and Ch-Put at the 2.5 mM concentration, suggesting the potential use of these substances in strategies to increase plant tolerance to chilling stress.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Reports

ISSN

2045-2322

e-ISSN

2045-2322

Volume of the periodical

15

Issue of the periodical within the volume

24 July

Country of publishing house

GB - UNITED KINGDOM

Number of pages

20

Pages from-to

26963

UT code for WoS article

001537443500013

EID of the result in the Scopus database

2-s2.0-105011539149