The identification of Armillaria species from soils in forest nurseries
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F07%3A00107563" target="_blank" >RIV/62156489:43410/07:00107563 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The identification of Armillaria species from soils in forest nurseries
Original language description
The genus Armillaria causes serious demage to forest stands in all Europe. In the Czech republic six species are presented, each of them differs in pathogenicity. In forest nurseries, however, no study aimed at armillaria were performed. Because armillaria do not fructificate at this age of trees, isolation of DNA from soil were chosen as the most suitable method. Our study took place at two different levels, as both bare-root seedlings and containers were tested for fungal DNA. The Armillaria specificprimer pair (AR1, AR2) based on conserved sequences within the so-called ITS region (ribosomal DNA) was used for direct amplification of DNA from soil samples by nested PCR. The individual species were distinguished by RFLPs analysis with restriction endonuclease Hinf I.
Czech name
Identifikace václavek rodu Armillaria z půd v lesních školkách
Czech description
Článek se zabývá izolací a následnou identifikací václavek rodu Armillaria z půd v lesních školkách pomocí metod molekulární genetiky. Konkrétně byla v tomto případě použita PCR-RFLP na ITS oblasti DNA za pomocí restrikční endonukleázy Hinf I.
Classification
Type
D - Article in proceedings
CEP classification
GK - Forestry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA526%2F05%2F0086" target="_blank" >GA526/05/0086: Armillaria: species, population ecology, phytopathology and economical importance on an example of Moravia and Silesia</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Kostelecké inspirování 2007, sborník abstraktů z konference 1. - 2. února 2007
ISBN
978-80-213-1624-9
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
21-21
Publisher name
ČZU Fakulta lesnická a environmentální
Place of publication
Praha
Event location
Kostelec nad Černými Lesy
Event date
Feb 1, 2007
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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