Development of microsatellite and mating type markers for the pine needle pathogen Lecanosticta acicola

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F14%3A00213818" target="_blank" >RIV/62156489:43410/14:00213818 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/article/10.1007/s13313-013-0256-5" target="_blank" >http://link.springer.com/article/10.1007/s13313-013-0256-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s13313-013-0256-5" target="_blank" >10.1007/s13313-013-0256-5</a>

Result language
angličtina
Original language name
Development of microsatellite and mating type markers for the pine needle pathogen Lecanosticta acicola
Original language description
Lecanosticta acicola is an ascomycete that causes brown spot needle blight of pine species in many regions of the world. This pathogen is responsible for a major disease of Pinus palustris in the USA and is a quarantine organism in Europe. In order to study the genetic diversity and patterns of spread of L. acicola, eleven microsatellite markers and two mating type markers were developed. An enrichment protocol was used to isolate microsatellite-rich DNA regions, and 18 primer pairs were designed to flank these regions, of which eleven were polymorphic. A total of 93 alleles were obtained across all loci from forty isolates of L. acicola from the USA with an allelic diversity range of 0.095 to 0.931 per locus. Cross-species amplification with some of the markers was obtained with L. gloeospora, L. guatemalensis and Dothistroma septosporum, but not with D. pini. Mating type (MAT) markers amplifying both idiomorphs were also developed to determine mating type distribution in populations.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/EE2.3.20.0265" target="_blank" >EE2.3.20.0265: Indicators of trees vitality</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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