Detection and quantification of airborne inoculum of Hymenoscyphus pseudoalbidus using real-time PCR assays

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F14%3A00228864" target="_blank" >RIV/62156489:43410/14:00228864 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/ppa.12218/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/ppa.12218/abstract</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/ppa.12218" target="_blank" >10.1111/ppa.12218</a>

Result language
angličtina
Original language name
Detection and quantification of airborne inoculum of Hymenoscyphus pseudoalbidus using real-time PCR assays
Original language description
A method based on real time PCR and using rotating arm spore traps was developed to quantify Hymenoscyphus pseudoalbidus airborne ascospores. The method was very sensitive and reproducible. The collection efficiency was evaluated to 10-15% of spores present in the air. The temporal ascospore dispersal pattern was studied over 3 years by collecting spores every 15 days during the ash growing season. The highest production was detected from the end of June to the beginning of September. The period of ascospore dispersal did not differ significantly among stands for a specific year. In contrast, there were differences according to the year. A positive correlation was calculated between the air temperature and the amount of ascospores trapped, with most ofthe positive samples observed above 12°C. The vertical profile of ascospore dispersal revealed a strong decrease in ascospore density within 3 m height regardless of the date of collection. Spore traps installed at increasing distances f
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GK - Forestry
OECD FORD branch
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Project
<a href="/en/project/EE2.3.20.0265" target="_blank" >EE2.3.20.0265: Indicators of trees vitality</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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