Effect of temperature on GaRV6 accumulation and its fungal host, the conifer pathogen Gremmeniella abietina

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F17%3A43909497" target="_blank" >RIV/62156489:43410/17:43909497 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/efp.12291" target="_blank" >http://dx.doi.org/10.1111/efp.12291</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/efp.12291" target="_blank" >10.1111/efp.12291</a>

Result language

angličtina

Original language name

Effect of temperature on GaRV6 accumulation and its fungal host, the conifer pathogen Gremmeniella abietina

Original language description

Gremmeniella abietina RNA virus 6 (GaRV6) was studied within the European race of G. abietina. We examined 97 isolates originating from Canada, Czech Republic, Finland, Italy, Montenegro, Serbia, Spain, Switzerland, Turkey and the United States. According to direct specific quantitative reverse transcription PCR (qRT-PCR) screening based on the RNA-dependent RNA polymerase (RdRp) sequence, the virus was mostly present in Spain, but it was also found at a very low concentration in three isolates from Canada, Italy and Finland. To gain insight into the three-way interaction among temperature, GaRV6 and G. abietina, we performed an in vitro experiment with eight Spanish isolates (four infected and four uninfected) with four repetitions each. We assessed the virus expression (accumulation) based on the quantity of RdRp-encoding RNA by qRT-PCR and the G. abietina growth rate at 5, 15 and 20oC. The presence of the virus had a significant negative effect on the G. abietina growth rate, which was also significantly influenced by the temperature. However, the temperature appeared not to clearly determine the virus accumulation, and the virus accumulation did not have any influence on the growth rate of the infected G. abietina isolates. Taken together, these results suggest that the role of this virus as a relevant factor with respect to the more thermophilic nature of the Spanish population of G. abietina may be ruled out.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

<a href="/en/project/EE2.3.20.0265" target="_blank" >EE2.3.20.0265: Indicators of trees vitality</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Forest Pathology

ISSN

1437-4781

e-ISSN

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Volume of the periodical

47

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

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UT code for WoS article

000397305800003

EID of the result in the Scopus database

2-s2.0-84978035459