Multiple Viral Infections Detected in Phytophthora condilina by Total and Small RNA Sequencing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F21%3A43919463" target="_blank" >RIV/62156489:43410/21:43919463 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.3390/v13040620" target="_blank" >https://doi.org/10.3390/v13040620</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/v13040620" target="_blank" >10.3390/v13040620</a>
Alternative languages
Result language
angličtina
Original language name
Multiple Viral Infections Detected in Phytophthora condilina by Total and Small RNA Sequencing
Original language description
Marine oomycetes have recently been shown to be concurrently infected by (MINUS SIGN )ssRNA viruses of the order Bunyavirales. In this work, even higher virus variability was found in a single isolate of Phytophthora condilina, a recently described member of Phytophthora phylogenetic Clade 6a, which was isolated from brackish estuarine waters in southern Portugal. Using total and small RNA-seq the full RdRp of 13 different potential novel bunya-like viruses and two complete toti-like viruses were detected. All these viruses were successfully confirmed by reverse transcription polymerase chain reaction (RT-PCR) using total RNA as template, but complementarily one of the toti-like and five of the bunya-like viruses were confirmed when dsRNA was purified for RT-PCR. In our study, total RNA-seq was by far more efficient for de novo assembling of the virus sequencing but small RNA-seq showed higher read numbers for most viruses. Two main populations of small RNAs (21 nts and 25 nts-long) were identified, which were in accordance with other Phytophthora species. To the best of our knowledge, this is the first study using small RNA sequencing to identify viruses in Phytophthora spp.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
<a href="/en/project/EF15_003%2F0000453" target="_blank" >EF15_003/0000453: Phytophthora Research Centre</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Viruses
ISSN
1999-4915
e-ISSN
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Volume of the periodical
13
Issue of the periodical within the volume
4
Country of publishing house
CH - SWITZERLAND
Number of pages
18
Pages from-to
620
UT code for WoS article
000643802200001
EID of the result in the Scopus database
2-s2.0-85105195930