Hidden Phytophthora diversity unveiled in tree nurseries of the Czech Republic with traditional and metabarcoding techniques

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F24%3A43925277" target="_blank" >RIV/62156489:43410/24:43925277 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1007/s10658-024-02886-1" target="_blank" >https://doi.org/10.1007/s10658-024-02886-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10658-024-02886-1" target="_blank" >10.1007/s10658-024-02886-1</a>

Result language

angličtina

Original language name

Hidden Phytophthora diversity unveiled in tree nurseries of the Czech Republic with traditional and metabarcoding techniques

Original language description

Phytophthora diversity was examined in eight forest and ornamental nurseries in the Czech Republic. A leaf baiting isolation technique and, in two nurseries, also Illumina DNA metabarcoding were used to reveal the diversity of Phytophthora in soil and irrigation water and compare the efficacy of both approaches. In total, baiting revealed the occurrence of 12 Phytophthora taxa in 59.4% of soil samples from seven (87.5%) nurseries. Additional baiting of compost was carried out in two nurseries and two Phytophthora species were recovered. Irrigation water was examined in three nurseries by baiting or by direct isolation from partially decomposed floating leaves collected from the water source, and two Phytophthora species were obtained. Illumina sequencing of soil and water samples was done in two and one nurseries, respectively. Phytophthora reads were identified as 45 Phytophthora taxa, 15 of them previously unknown taxa from Clades 6, 7, 8 and 9. Another 11 taxa belonged to known or undescribed species of the oomycete genera Globisporangium, Hyaloperonospora, Nothophytophthora, Peronospora and Plasmopara. Overall, with both techniques 50 Phytophthora taxa were detected with five taxa (P. taxon organica, P. plurivora, P. rosacearum, P. syringae and P. transitoria) being exclusively detected by baiting and 38 only by DNA metabarcoding. Particularly common records in DNA barcoding were P. cinnamomi and P. lateralis which were not isolated by baiting. Only seven species were detected by both techniques. It is recommended to use the combination of both techniques to determine true diversity of Phytophthora in managed or natural ecosystems and reveal the presence of rare or unknown Phytophthora taxa.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10612 - Mycology

Project

<a href="/en/project/EF15_003%2F0000453" target="_blank" >EF15_003/0000453: Phytophthora Research Centre</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Journal of Plant Pathology

ISSN

0929-1873

e-ISSN

1573-8469

Volume of the periodical

170

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

26

Pages from-to

131-156

UT code for WoS article

001250018300002

EID of the result in the Scopus database

2-s2.0-85196077697