DNA-methylation changes in grapevine somaclones following in vitro culture and thermotherapy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F10%3A00166562" target="_blank" >RIV/62156489:43510/10:00166562 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
DNA-methylation changes in grapevine somaclones following in vitro culture and thermotherapy
Original language description
The methylation-sensitive amplified polymorphism (MSAP) technique using HpaII and MspI isoschizomers was used to analyse DNA-methylation alterations in stressed grapevine plants. The stress used was in vitro propagation via nodal segments and in vitro thermotherapy for virus elimination.Clustering of variants within resulting dendrograms showed significant differences between woody cuttings despite originating from the one plant. Further, there was a strong 'donor' effect of maternal plants on future arrays of DNA methylation in their regenerants. The 'donor' effect even seemed to prevail in the effect of stress on final DNA-methylation state in stressed regenerants.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/OC%20116" target="_blank" >OC 116: Effect of in vitro manipulations and thermotherapy-induced stress on genetic and epigenetic changes in the grapevine genome</a><br>
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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