Evaluation of the AFLP and MSAP methods as tools for studying of DNA changes in grapevine plants long period after their in vitro thermotherapy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F12%3A00198664" target="_blank" >RIV/62156489:43510/12:00198664 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Evaluation of the AFLP and MSAP methods as tools for studying of DNA changes in grapevine plants long period after their in vitro thermotherapy

Original language description

To evaluate the impact of the stress induced by thermotherapy on the primary structure of DNA, AFLP (Amplified Fragment Length Polymorphism) was applied in this study. Epigenetic changes were determined by means of MSAP (Methylation Sensitive Amplified Polymorphism). Studies were carried out on plants at different times after thermotherapy treatments (6 weeks to 5 years after it). The results show nearly identical AFLP-derived fingerprints in all analysed samples from plants treated by in vitro thermotherapy, suggesting that the stress condition did not provoke changes at the sequence level or the method is not suitable to detect possible changes. In contrast, MSAP allowed the observation of differences in epigenetic fingerprints in thermotherapy treated plants. Furthermore, the method allowed to determine the impact in a time dependent and quantifiable fashion. Plants sampled 6 weeks after thermotherapy showed most different epigenetic profiles, whereas plants with longer period after

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

<a href="/en/project/QH91153" target="_blank" >QH91153: Exploitation of in vitro cultures for recovering of cultivars of fruit trees and grapevines from viruses, phytoplasmas and quarantine pathogens for the system of certification of planting material including verification of quality.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Horticulturae

ISSN

0567-7572

e-ISSN

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Volume of the periodical

961

Issue of the periodical within the volume

1

Country of publishing house

BE - BELGIUM

Number of pages

8

Pages from-to

73-80

UT code for WoS article

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EID of the result in the Scopus database

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