An evaluation of the impact of demethylating agents treatment using TGS 16C nicotiana benthamiana reporter line
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F15%3A43906742" target="_blank" >RIV/62156489:43510/15:43906742 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
An evaluation of the impact of demethylating agents treatment using TGS 16C nicotiana benthamiana reporter line
Original language description
Epigenetics is one of the fastest-growing areas of science and has now become a central issue in biological studies of development, stress impact and disease. DNA methylation provides a way how to alter the gene expression pattern without disrupting or modifying the genome. Here, we demonstrate the potential of a Nicotiana benthamianaTGS 16C reporter line in the signalization of demethylating events caused by the activity of the so-called demethylating agents. These compounds have the ability to block or to interfere with the activity of methyltransferases, enzymes responsible for maintaining methylation marks on the replicating sequences. This N. benthamianaline carries a green fluorescent protein gene (GFP), whose promoter had been methylated and isthus inactive. By treating such plants with compounds with demethylating properties, their demethylating potential can be estimated by the effect on the re-established GFP expression in plant tissues.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2015: Proceedings of International PhD Students Conference
ISBN
978-80-7509-363-9
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
428-433
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 11, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000366466100080