Comparison of real-time PCR protocols in detection and quantification of fruit tree 16SrX group phytoplasmas
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F16%3A43909732" target="_blank" >RIV/62156489:43510/16:43909732 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.2298/GENSR1602629K" target="_blank" >http://dx.doi.org/10.2298/GENSR1602629K</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2298/GENSR1602629K" target="_blank" >10.2298/GENSR1602629K</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of real-time PCR protocols in detection and quantification of fruit tree 16SrX group phytoplasmas
Original language description
In this work, two real-time PCR protocols based on intercalating dye and two on hydrolysis probes were tested using field collected fruit tree samples infected by 16SrX group (AP, PD and ESFY) phytoplasmas. Specificity and sensitivity of protocols and amplification efficiency were the main testing parameters. Results of real-time PCR protocols were compared to nested PCR. All real-time PCR protocols confirmed their specificity of detection. All real-time PCR protocols were 10-100 times more sensitive than nested PCR. Afterall real-time PCR protocols based on hydrolysis probes were 10 times more sensitive than protocols based on intercalating dyes. Among protocols based on hydrolysis probes, slightly better detection characteristics were shown by protocol by CHRISTENSEN et al. (2004).
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GF - Diseases, pests, weeds and plant protection
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/QJ1510352" target="_blank" >QJ1510352: Assessment of the factors influencing harmful phytoplasmas invading fruit trees and verifying effective means of their elimination</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Genetika
ISSN
0534-0012
e-ISSN
—
Volume of the periodical
48
Issue of the periodical within the volume
2
Country of publishing house
CS - SERBIA AND MONTENEGRO
Number of pages
14
Pages from-to
629-642
UT code for WoS article
000389658100016
EID of the result in the Scopus database
2-s2.0-84994517482