Grapevine Trunk Diseases Pathogens Identification on Grapevine Rootstocks in Ukraine

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F19%3A43916713" target="_blank" >RIV/62156489:43510/19:43916713 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.15407/microbiolj81.02.065" target="_blank" >https://doi.org/10.15407/microbiolj81.02.065</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15407/microbiolj81.02.065" target="_blank" >10.15407/microbiolj81.02.065</a>

Result language

angličtina

Original language name

Grapevine Trunk Diseases Pathogens Identification on Grapevine Rootstocks in Ukraine

Original language description

The objective of this study was isolation of fungi from the grapevine trunks, DNA extraction and identification of potential esca pathogens in Ukraine. Methods. Isolation and identification of fungal cultures based on morphology and DNA sequencing. Total DNA of fungal colonies was extracted by NucleoSpin Tissue kit (Macherey-Nagel, Düren, Germany) according to manufacturer&apos;s instructions. To amplify ITS region, ITS1 and ITS4 primers were used. The PCR products corresponding to the size of approx. 550 bp. were sequenced and the obtained nucleotide sequences were analysed using CLC Main Workbench 5.0 (CLC bio, Aarhus, Denmark). Results. Determination of potential pathogens complex showed the presence of species associated with &quot;trunk diseases&quot;. Among specific esca pathogens 3 isolates of Cadophora luteo-olivacea were found out. Esca-affected samples also showed the presence of Eutypa lata (causal agent of Eutypa dieback) and Botryosphaeria dothidea, (causal agent of Botrioshaeria dieback). Conclusions. DNA identification of fungal pathogens showed a discrepancy in species composition between asymptomatic plants and esca-affected plants. Esca-affected cultivars showed the presence of Eutypa lata and Botryosphaeria dothidea, which were absent in asymptomatic plants.

Czech name

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Czech description

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Type

J_SC - Article in a specialist periodical, which is included in the SCOPUS database

CEP classification

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OECD FORD branch

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mìkrobiologičnij Žurnal

ISSN

1028-0987

e-ISSN

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Volume of the periodical

81

Issue of the periodical within the volume

2

Country of publishing house

UA - UKRAINE

Number of pages

8

Pages from-to

65-72

UT code for WoS article

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EID of the result in the Scopus database

2-s2.0-85075170209