Pseudoperoxidase activity of silver nanoparticles (AgNPs) and changes in peroxidase activity in maize plants exposed to AgNPs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F20%3A43917988" target="_blank" >RIV/62156489:43510/20:43917988 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.37904/nanocon.2019.8511" target="_blank" >https://doi.org/10.37904/nanocon.2019.8511</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2019.8511" target="_blank" >10.37904/nanocon.2019.8511</a>

Result language

angličtina

Original language name

Pseudoperoxidase activity of silver nanoparticles (AgNPs) and changes in peroxidase activity in maize plants exposed to AgNPs

Original language description

Some types of nanoparticles such as gold and silver show interesting chemical properties. Pseudoperoxidase activity has been found in iron- or gold-based materials, but some silver nanoparticles (AgNPs) also exhibit such properties. Pseudoperoxidase activity of AgNPs prepared by green synthesis using sage leaves was studied. The AgNPs (prepared by green synthesis using sage leaves) size ranged from 20 to 50 nm and zeta potential was found to vary from -10 to 20 mV, with absorption maximum being 430 nm. The peroxidase-like activity was monitored in the presence of 5 mM 3,3&apos;,5,5&apos;-tetramethylbenzidine (TMB) and hydrogen peroxide (30%). The nanoparticles showed about 80% activity of 1 mU horseradish peroxidase (HRP). In addition, AgNPs were applied to maize seedlings (5 days in hydroponics system) at concentrations of 1 mg/L, 50 mg/L, and 150 mg/L. Peroxidase activity in leaves after 48 hours was 59.5, 67.5, and 57.2 µg.mL-1 of HRP, respectively. Peroxidase activity of untreated plant leaves was 58.8 µg.mL-1 of HRP. Peroxidase activity in roots after 48 hours was 101.6, 109.0, and 156.7 µg.mL-1 of HRP, respectively. The peroxidase activity level of the control variant in root of treatment was 98.7 µg.mL-1 of HRP. The results indicate that the determination of peroxidase activity could be used to assess the phytotoxicity of nanoparticles in plants.

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

—

OECD FORD branch

21001 - Nano-materials (production and properties)

Project

<a href="/en/project/LTC18002" target="_blank" >LTC18002: The development of new materials suitabled for 3D printing with antimicrobial properties (3D ANTIMICROB)</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NANOCON 2019: Conference Proceedings

ISBN

978-80-87294-95-6

ISSN

2694-930X

e-ISSN

—

Number of pages

6

Pages from-to

342-347

Publisher name

Tanger Ltd.

Place of publication

Ostrava

Event location

Brno

Event date

Oct 16, 2019

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000664115400056