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The use of SSRs for the identification of unknown Asian pear cultivars

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F21%3A43919605" target="_blank" >RIV/62156489:43510/21:43919605 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.17660/ActaHortic.2021.1307.32" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2021.1307.32</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.17660/ActaHortic.2021.1307.32" target="_blank" >10.17660/ActaHortic.2021.1307.32</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    The use of SSRs for the identification of unknown Asian pear cultivars

  • Original language description

    Pear species belong to one of the longest-cultivated temperate species. A large number of cultivars is spread worldwide, that have different names in different countries. Probably the most famous is the cultivar &apos;Williams&apos;, which is known in the USA as &apos;Bartlett&apos;. However, not all synonyms are always known. Molecular methods, namely the SSR method, allow reliable identification of cultivars. In this study, an SSR marker set was applied to 31 Asian pear cultivars, 4 interspecific hybrids and 3 botanical species from the East China gene center. Six SSR primer pairs were used to determine the genetic relatedness. SSR primers were labeled with fluorescent dyes and the PCR products were evaluated using a genetic analyzer. Based on the obtained results, the cultivars were divided into two main clusters. Cluster I was divided into three subclusters, subcluster I contained P. pyrifolia and cultivars derived therefrom, subcluster II contained P. ussuriensis and several ussuriensis-type cultivars and Chinese white pear cultivars and subcluster III contained cultivars of the Chinese white pears. Cluster II was formed by only 4 cultivars probably being interspecific hybrids. Further, combinations of cultivars &apos;Man San Gill&apos; and &apos;Pung Su&apos;; &apos;Early&apos; and &apos;Zaosu Li&apos;; &apos;Snow Flower&apos;, &apos;Shon Shu&apos; and &apos;Dangshanshu Li&apos; had identical SSR profiles, which indicates, that they are probably identical cultivars or possibly different clones.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    40105 - Horticulture, viticulture

Result continuities

  • Project

    <a href="/en/project/QK1910137" target="_blank" >QK1910137: Aplication of new fruit species for long-term maintenance of fruit production potential in conditions of climate changing</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Acta Horticulturae 1307

  • ISBN

    978-94-6261-306-5

  • ISSN

    0567-7572

  • e-ISSN

    2406-6168

  • Number of pages

    7

  • Pages from-to

    205-211

  • Publisher name

    International Society for Horticultural Science (ISHS)

  • Place of publication

    Leuven

  • Event location

    Praha

  • Event date

    Jun 3, 2019

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article

    000948137200031