LIGHT MICROSCOPIC AND FLOW CYTOMETRIC DETECTION OF APOPTOSIS AND NECROSIS OF NEUTROPHILS IN THE MAMMARY GLAND OF THE VIRGIN HEIFER
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F01%3A23100002" target="_blank" >RIV/62156489:_____/01:23100002 - isvavai.cz</a>
Alternative codes found
RIV/00027162:_____/01:80000025
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
LIGHT MICROSCOPIC AND FLOW CYTOMETRIC DETECTION OF APOPTOSIS AND NECROSIS OF NEUTROPHILS IN THE MAMMARY GLAND OF THE VIRGIN HEIFER
Original language description
Sládek Z., D. Ryšánek, M. Faldyna: Light Microscopic and Flow Cytometric Detection of Apoptosis and Necrosis of Neutrophils in the Mammary Gland of the Virgin Heifer. Acta Vet. Brno 2001, 70: 149-155. FIow cytometry analysis of discrimination of apoptotic and necrotic neutrophils and their distribution on dot plots using cells influx induced by intramammary administration of buffered saline was verified by light microscopy .Twenty mammary glands of five nonpregnant heifers were used and samples of mammary gland lavages were collected before (control) and 24, 48, 72, and 96 h after treatment and examined by Iight microscopy and flow cytometry with Annexin V and propidium iodide staining. Verification of flow cytometry method was provided by morphological features of leukocytes in light microscopy and by changes in differentia1 cell counts during induced influx. Detection of apoptotic and necrotic neutrophils was made using backgating technique.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GG - Zootechnics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA524%2F01%2F1290" target="_blank" >GA524/01/1290: Effects of antimicrobial agens on induction of apoptosis and necrosis of polymorphonuclear leukocytes during resolution of bovine mastitis</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACTA VETERINARIA BRNO
ISSN
0001-7213
e-ISSN
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Volume of the periodical
70
Issue of the periodical within the volume
leden
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
7
Pages from-to
149-155
UT code for WoS article
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EID of the result in the Scopus database
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