The cloning and use of pGREEN based vecttors for the transformation of Pea (Pisum sativum L.)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F03%3A21100011" target="_blank" >RIV/62156489:_____/03:21100011 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The cloning and use of pGREEN based vecttors for the transformation of Pea (Pisum sativum L.)
Original language description
Transformation is emerging as an important crop improvement tool and the engineering of crops with specific traits can be achieved. Among others the transformation of plants with cDNA of viral genes has been shown to result in resistance of the plants tothe corresponding virus. The pGreen series of binary Ti vectors are configured for easy manipulation and to meet the demands of a wide range of transformation procedures for many plant species. Further we optimised the transformation method of pea and the best results were obtained by the sonication-assisted Agrobacterium-mediated transformation of pea embryonic segments. After finishing of the screening of pea virus diseases in field conditions in the Czech republic the appropriate virus gene cDNAs will be cloned into the tested vectors and used for pea transformation.
Czech name
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Czech description
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Project
<a href="/en/project/QF3072" target="_blank" >QF3072: Development of transgenic pea (Pisum sativum L.) lines with enhanced resistance to viral pathogens.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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