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Possibilities of using the PCR - RFLP method for intraspecific differentiation between Sus scrofa scrofa and Sus scrofa domesticus.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A_____%2F03%3A42400021" target="_blank" >RIV/62156489:_____/03:42400021 - isvavai.cz</a>

  • Alternative codes found

    RIV/62157124:16270/03:00000385

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Possibilities of using the PCR - RFLP method for intraspecific differentiation between Sus scrofa scrofa and Sus scrofa domesticus.

  • Original language description

    This report focuses on the possibilities of differentiating pig and wild boar meat by molecular biological methods in the DNA polymorphism analysis. On the basis of published polymorphisms, the PCR-RFLP method was used in the parts of the mtDNA gene forcytochrome b and the D-loop region in 33 samples of wild boar and 10 samples of domestic pig. Intraspecific polymorphism was only found in the D-loop region of mtDNA. An amplified 531 bp-long PCR fragment was digested with restriction endonuclease Tsp509I. Based on restriction fragment length analysis, the domestic pig samples were divided into two groups, and the wild boar samples into three. The polymorphisms found enabled intraspecific differentiation in the species Sus scrofain

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    GK - Forestry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2003

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Fleischwirtschaft

  • ISSN

    0179-2415

  • e-ISSN

  • Volume of the periodical

    2

  • Issue of the periodical within the volume

    D42804F

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    3

  • Pages from-to

    39-41

  • UT code for WoS article

  • EID of the result in the Scopus database