The Production and Application of Single-chain Antibody Fragments
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F03%3A00000193" target="_blank" >RIV/62157124:16170/03:00000193 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The Production and Application of Single-chain Antibody Fragments
Original language description
The possibility of routine production of monoclonal antibodies (mAbs) in hybridomas has opened new horizons in many research fields. However, advances in molecular biology made possible further manipulations with antibody genes, construction of antibodylibraries and creation of sophisticated antibody selection systems. Thus, recombinant monoclonal antibodies were generated, and research and the practical applications of mAbs were extended. In this review, we will focus mainly on one category of recombinant antibodies, the single-chain antibody fragment (scFv). ScFv consists of only variable regions of heavy and light chains connected with a linker sequence. This antibody fragment retains specificity, is encoded by a single gene and expressed as a single polypeptide chain, and can be generated from either hybridomas or phage and ribosomal display libraries. Simple manipulation and versatile use made scFv the most popular recombinant antibody format. We will briefly discuss methods for
Czech name
Příprava a aplikace rekombinantních protilátek
Czech description
The possibility of routine production of monoclonal antibodies (mAbs) in hybridomas has opened new horizons in many research fields. However, advances in molecular biology made possible further manipulations with antibody genes, construction of antibodylibraries and creation of sophisticated antibody selection systems. Thus, recombinant monoclonal antibodies were generated, and research and the practical applications of mAbs were extended. In this review, we will focus mainly on one category of recombinant antibodies, the single-chain antibody fragment (scFv). ScFv consists of only variable regions of heavy and light chains connected with a linker sequence. This antibody fragment retains specificity, is encoded by a single gene and expressed as a single polypeptide chain, and can be generated from either hybridomas or phage and ribosomal display libraries. Simple manipulation and versatile use made scFv the most popular recombinant antibody format. We will briefly discuss methods for
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA524%2F01%2F0819" target="_blank" >GA524/01/0819: Inhibition of Maedi-Visna Virus Replication by Intracellular Immunisation with scFv derived intrabodies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Folia Microbiologica
ISSN
0015-5632
e-ISSN
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Volume of the periodical
48
Issue of the periodical within the volume
5
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
12
Pages from-to
687-698
UT code for WoS article
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EID of the result in the Scopus database
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