Conception rate after sex determination and cryopreservation of D7 bovine embryos

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F10%3A00003003" target="_blank" >RIV/62157124:16170/10:00003003 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Conception rate after sex determination and cryopreservation of D7 bovine embryos

Original language description

We evaluated conception rate after sex determination and subsequent freezing of D 7 bovine embryos and compared it with transfer of fresh female embryos. Fresh embryos were blade biopsied (n=172) and then transferred. High quality embryos (n=112) were biopsied by cell aspiration and cryopreserved. Sex determination (PCR using primers for the Y-chromosome determinant) was successfully completed in 91.3% of fresh (44% female) and in 87.5% of freeze-thawed embryos (45.9% female). The pregnancy rate was 56.5% after fresh transfer of sexed (female) embryos whereas intact embryos (control 1) implanted in 61.9% recipients. 95.6% (43/45) of embryos survived biopsy and cryopreservation. Pregnancy rate after transfer of freeze-thawed sexed (female) embryos was 48.8%. A similar conception rate was found in intact embryos (50.7%, control 2). The results demonstrate that these techniques allow the rapid commercial exchange of genetic resources on the basis of fresh or frozen sex-desired embryos in

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Volume of the periodical

55

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

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UT code for WoS article

000275478200002

EID of the result in the Scopus database

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