Expression and serological reactivity of Nsp7 protein of PRRS genotype I virus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F12%3A43871160" target="_blank" >RIV/62157124:16170/12:43871160 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16810/12:43871688
Result on the web
<a href="http://dx.doi.org/10.1016/j.rvsc.2012.06.007" target="_blank" >http://dx.doi.org/10.1016/j.rvsc.2012.06.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.rvsc.2012.06.007" target="_blank" >10.1016/j.rvsc.2012.06.007</a>
Alternative languages
Result language
angličtina
Original language name
Expression and serological reactivity of Nsp7 protein of PRRS genotype I virus
Original language description
The aim of this work was to express recombinant nonstructural Nsp7 protein of European genotype of porcine reproductive and respiratory syndrome virus and to evaluate its diagnostic sensitivity and specificity in serological diagnostics of the disease. The gene coding for Nsp7 protein was expressed in Escherichia coli cells and purified by IMAC. Serological reactivity of purified protein was assessed on a panel of swine sera in indirect ELISA test. Serum samples originated from PRRS positive farms, herds free of PRRS infection and PRRS free herds vaccinated with an inactivated vaccine. Nsp7 antigen proved to be suitable for serological detection of PRRS specific antibodies, showing diagnostic sensitivity of 82.2% and specificity of 97.6% when comparedwith IDEXX ELISA test. The nonstructural protein proved to be suitable for use as an antigen for the differentiation of post-infection and post-vaccination antibodies in pigs vaccinated with the inactivated vaccine. But the low overall an
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GJ - Diseases and animal vermin, veterinary medicine
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Research in Veterinary Science
ISSN
0034-5288
e-ISSN
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Volume of the periodical
93
Issue of the periodical within the volume
3
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
1537-1542
UT code for WoS article
000311185900077
EID of the result in the Scopus database
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