Sequence analysis of selected nucleotide sequences of abortogenic isolate of Equine herpesvirus 1 and changes caused by serial passage in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F12%3A43871635" target="_blank" >RIV/62157124:16170/12:43871635 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.2754/avb201281010009" target="_blank" >http://dx.doi.org/10.2754/avb201281010009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201281010009" target="_blank" >10.2754/avb201281010009</a>

Result language

angličtina

Original language name

Sequence analysis of selected nucleotide sequences of abortogenic isolate of Equine herpesvirus 1 and changes caused by serial passage in vitro

Original language description

The aim of this work was to isolate the abortogenic virus strain of equine herpesvirus 1 representing the current infection situation in the Czech Republic, describe it at the molecular level with the accent to genes coding viral glycoproteins and observe the changes caused by passaging of the virus on a cell culture. In 2009, an isolate of equine herpesvirus 1 was obtained from an abortion case in a mare from a herd affected by an abortion storm. The virus identification was performed using the PCR method. The virus was isolated on the RK 13 cell line and after 6 passages in vitro the stability of sequences of selected sections of the virus genome was assessed and compared with the original field isolate. The virus sequences were also compared with known sequences of the abortogenic reference virus strain (V592) and with other known viral strains. One point mutation in a nucleotide sequence coding glycoprotein G was found, distinguishing the field isolate from V592. One point mutation

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GJ - Diseases and animal vermin, veterinary medicine

OECD FORD branch

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Project

<a href="/en/project/QH92265" target="_blank" >QH92265: Optimization of breeding system and health care in endangered breed of Old Kladruby horse</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

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Volume of the periodical

81

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

9-13

UT code for WoS article

000305600200002

EID of the result in the Scopus database

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